Correlative light-ion microscopy for biological applications

microscopy and analysis Correlative Light and Electron Microscopy

Bridging microscopes: 3D correlative light and scanning electron microscopy of complex biological structures.

The rationale of correlative light and electron microscopy (CLEM) is to collect data on different information levels--ideally from an identical area on the same sample--with the aim of combining datasets at different levels of resolution to achieve a more holistic view of the hierarchical structural organization of cells and tissues. Modern three-dimensional (3D) imaging techniques in light and...

Preparation of Biological Samples for Ion Microscopy

Ion Microscopy is a powerful technique for intracellular elemental localization in b specimens. The effechve w of this technique depends on the reliability of sample preparation. An ideal Sam le reparation should preserve the chemical and structural integrity of a living cell, and it shouPd ago satisfy the instrumental requirements for analysis. This article critically reviews different methods...

Correlative Light and Electron Microscopy (CLEM) and its applications in infectious disease

Correlative light and electron microscopy (CLEM) is an effective technique used to study biological samples. Signal-specific indicators observable under light microscopy (LM) allow scientists to locate areas of interest for high resolution ultra-structure observation under the electron microscope (EM). Recent method developments provided breakthroughs creating an effective, direct, and accurate...

Correlative Light Electron Microscopy: Connecting Synaptic Structure and Function

Many core paradigms of contemporary neuroscience are based on information obtained by electron or light microscopy. Intriguingly, these two imaging techniques are often viewed as complementary, yet separate entities. Recent technological advancements in microscopy techniques, labeling tools, and fixation or preparation procedures have fueled the development of a series of hybrid approaches that...